A successful dental management in a child with Cornelia de Lange Syndrome: rare case of Cornelia de Lange Syndrome in child / O sucesso do manejo odontológico em criança com Síndrome Cornelia de Lange: um caso raro da Síndrome Cornelia de Lange em criança

Background: the Cornelia de Lange Syndrome (CDLs) is a rare and complex syndrome characterized, basically, by psychomotor retardation associated with a number of congenital malformations. Aims: this paper reports the case of an 11-year-old female child diagnosed with Cornelia de Lange Syndrome (CdLS) and her successful dental management. Case report: the patient had severe mental retardation, definite negative behavior and the clinical findings included oral and physical changes. The patient's oral hygiene was deficient with the presence of calculus and gingivitis, besides several active caries lesions in permanent and deciduous dental elements. The treatment consisted in guidance for caregivers about oral hygiene and diet, and the dental procedures were performed under general anesthesia. Currently, the patient is accompanied by monthly follow-ups. Conclusions: the lack of knowledge about oral hygiene and cariogenic diets was identified as a one of the reasons for the oral diseases present. Due to the need to care for the other more serious and complex health problems, the oral diseases had evolved faster than usual and thus were difficult to treat and maintain thereafter. Under such conditions, the dentist plays a key role within a multidisciplinary team. From the guidance and knowledge provided in the dental clinic, there was a significant improvement in the life quality of the child and her family

Identification of Enterococcus faecalis by PCR in saliva of pediatric patients / Identificação do Enterococcus faecalis por PCR em saliva de pacientes pediátricos

This study aimed to identify E. faecalis in saliva of patients that were divided into two groups: 10 patients with caries lesions and 10 caries-free patients. Material and methods: Saliva samples were collected with a sterile swab and inoculated in enterococcosel media for 48 hours. The positive samples were subcultured in broth agar - blood medium for storage and subsequent PCR analysis. The data were analyzed using the SPSS (÷2). Results: From the 20 cases included in the study, 3 were positive in the Enterococcosel medium, and both tests (culture and PCR) used confirmed that three of them belonged to the species E. faecalis. No samples were positive in Enterococcosel broth in the group of caries-free patients. From the three samples that were identified as Enterococcus in broth (positive bile esculin test), an amplified for E. faecalis PCR analysis (p > 0.005). Conclusion: Through the use of PCR, it was possible to identify the genus Enterococcus and the species E. faecalis in saliva of patients with carious lesions – the pathogen that may influence the prognosis of diseases of the oral cavity...

Este estudo objetivou identificar E. faecalis em saliva de pacientes que foram divididos em dois grupos: 10 pacientes com lesões de cárie e 10 livres de cárie. Material e métodos: Amostras de saliva foram coletadas com um swab estéril e inoculadas em meios Enterococcosel por 48 horas. As amostras positivas foram repicadas em meio de caldo de agar - sangue para armazenamento e análise de PCR subsequente. Os dados foram analisados utilizando o SPSS. Resultados: Dos 20 casos incluídos no estudo, três foram positivos no médio Enterococcosel e ambos os testes (cultura e PCR) utilizados confirmaram que três deles pertenciam à espécie E. faecalis. Nenhuma amostra foi positiva em caldo Enterococcosel no grupo de pacientes livres de cáries. A partir das três amostras que foram identificadas como Enterococcus em caldo (teste positivo esculina biliar), foram amplificados por análise de PCR para o E. faecalis (p>0.005). Conclusão: Através da utilização de PCR, foi possível identificar gênero Enterococcus e a espécie de E. faecalis em saliva de pacientes com lesões de cárie – o agente patogênico que pode influenciar o prognóstico de doenças da cavidade oral...